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Journal: Materials Today Bio
Article Title: Intravesical folate-conjugated hydroxyethyl starch micelles for pH-triggered co-delivery of epirubicin and TLR7 agonist toward synergistic chemoimmunotherapy of bladder cancer
doi: 10.1016/j.mtbio.2026.102835
Figure Lengend Snippet: (A) CLSM images of RAW 264.7 cells after treatment with LPS + IFN-γ and IL-4. Cell nuclei were stained with DAPI (blue), CD86 fluorescence displayed in green and CD206 fluorescence displayed in red. Scale bar: 10 μm; (B) Confocal imaging of CD86 and CD206 expression in M2-TAMs treated with free IMQ, FA-HES-EPI, and FA-HES-EPI/IMQ micelles. Cell nuclei were stained with DAPI (blue), CD86 fluorescence displayed in green and CD206 fluorescence displayed in red. Scale bar: 10 μm; (C) FCM analysis of expression of CD86 and CD206 after co-culturing M2-TAMs with different formulations; (D) Mean fluorescence intensity of CD86 and CD206 in M2-TAMs treated with different formulations. (n = 3), n.s.: p > 0.05, **p < 0.01and ****p < 0.0001 vs. the IL-4 group; ##p < 0.01and ####p < 0.0001 vs. the IL-4 group; (E) Expression levels of IL-6 and TNF-α in RAW264.7 cells treated with free IMQ and FA-HES-EPI/IMQ micelles. (n = 3), n.s.: p > 0.05, ***p < 0.001 and ****p < 0.0001; ####p < 0.0001; (F) Western blot analysis of TLR7/MyD88/NF-κB signaling in RAW 264.7 cells treated with free IMQ or FA-HES-EPI/IMQ micelles.
Article Snippet: Rabbit anti-CD86 polyclonal antibody, rabbit anti-CD206 polyclonal antibody, FITC goat anti-rabbit antibody, IgG/Alexa Fluor555 goat anti-rabbit antibody, APC anti-mouse CD206 antibody,
Techniques: Staining, Fluorescence, Imaging, Expressing, Western Blot